Determination of effective diffusion coeficient of immobilized baker's yeast invertase in various concentration of PVA-alginate matrix

Abstract

Baker's yeast invertase is immobilizzed in PVA-alginate matrix using an improved method PVA beads were prepared by adding calcium alginate to improve its stability, mechanical and chemical properties. Boric acid was used as the cross-linking agent and additional chemicals consisteing of 10% boric acid and sodium sulphate solution was used as a treatment solution to harden the PVA-alginate beads. The determination of the effective diffusion of PVA-alginate matrix is the vital step in optimizing the preparation of immobilized and water-soluble biocatalyst. In the study the two-level full factorial design was used to investigate the effect of PVA and boric acid concentrations on diffusion coefficient.