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Mangosteen (garciniamangostanal.) leaves extract increased melanogenesisin B16F1 melanoma cell line

Abdul Hamid, Mariani and Sarmidi, Mohamad Roji and Chang, Seo Park (2010) Mangosteen (garciniamangostanal.) leaves extract increased melanogenesisin B16F1 melanoma cell line. In: The Korean Society for Biotechnology and Bioengineering Spring Meeting & International Symposium, April 15-16, 2010, Kyungwon University, South Korea.

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Official URL: http://www.earticle.net/Article.aspx?sn=119848


Melanogenesis is a multistage process involving melanin synthesis, melanin transport and melanosome release. Melanin synthesis is stimulated by various effectors, including α-melanocyte-stimulating hormone (α-MSH), cyclic AMP (cAMP)-elevating agents (forskolin, isobutylmethylxantine, and glycyrrhizin) and ultraviolet light. In this study, the water extract from mangosteen (garcinia mangostana) leaves was assessed with regard to its effects on melanogenesis in B16F1 melanoma cells line. The mangosteen leaves extract was shown to stimulate melanin synthesis and tyrosinase activity in a dose-dependent manner without any significant effects on cell proliferation. Formation of melanin from cultured B16F1 melanoma induced by mangosteen leaves extract treatment was estimated using spectrophotometer. In the range of non-cytotoxic concentrations at 7.8x10-4 % ~ 6.2x10-3 % (w/v) mangosteen leaves extract strongly increased melanogenesis. The results obtained clearly shows that mangosteen leaves extract increase melanogenesis almost 2.5 folds compare to the α-MSH control group. In order to clarify the further mechanism of tyrosinase activation by mangosteen leaves extract, the level of tyrosinase expression in B16F1 cells were examine by intracellular tyrosinase assay and tyrosinase zymography. The result showed that tyrosinase activity was markedly enhanced from mangosteen treated cells. These results suggested that mangosteen leaves extract might be one of the promising candidates in order to treat hypo pigmentation disorder and useful for self tanning cosmetic products.

Item Type:Conference or Workshop Item (Paper)
Uncontrolled Keywords:melanogenesis, mangosteen, tyrosinase
Subjects:T Technology > TP Chemical technology
Divisions:Chemical and Natural Resources Engineering (Formerly known)
ID Code:24082
Deposited By: Mrs Liza Porijo
Deposited On:27 Sep 2012 03:28
Last Modified:05 Sep 2017 07:24

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