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Liquid state bioconversion of palm oil mill effluent for cellulase production: statistical optimization of process conditions

Alam, Md. Zahangir and Abd Karim, M. Ismail and Hamisan, Ahmad Fadhlan and Jamal, Parveen and Jalal, K.C.A. (2006) Liquid state bioconversion of palm oil mill effluent for cellulase production: statistical optimization of process conditions. In: 1st International Conference on Natural Resources Engineering & Technology 2006, 24-25th July 2006, Putrajaya, Malaysia.

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Abstract

The filamentous fungus Trichoderma harzianum was used for liquid state bioconversion of POME for cellulase production. Statistical optimization was carried out to evaluate the physico-chemical parameters (factors) for maximum cellulase production by 2-level fractional factorial design with six central points. The polynomial regression model was developed using the experimental data including the effects of linear, quadratic and interaction of the factors. The factors involved were substrate (POME) and co-substrate (wheat flour) concentrations, temperature, pH, inoculum and agitation. Statistical analysis showed that the optimum conditions were: temperature of 300C, substrate concentration of 2%, wheat flour concentration of 3%, pH of 4, inoculum of 3% and agitation of 200 rpm. Under these conditions, the model predicted the enzyme production to be about 14 FPU/ml. Analysis of variance (ANOVA) of the design showed a high coefficient of determination (R2) value of 0.99, thus ensuring a high satisfactory adjustment of the quadratic model with the experimental data.

Item Type:Conference or Workshop Item (Paper)
Uncontrolled Keywords:Palm oil mill effluent; cellulase enzyme; bioconversion; statistical optimization
Subjects:T Technology > TP Chemical technology
Divisions:Chemical and Natural Resources Engineering (Formerly known)
ID Code:234
Deposited By: Cik Rasyidah Mohd Daud
Deposited On:15 Feb 2007 10:48
Last Modified:01 Jun 2010 02:41

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