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Comparison of different extraction methods for the extraction of major bioactive flavonoid compounds from spearmint (Mentha spicata L.) leaves

Bimakra, Mandana and Abdul Rahmana, Russly and Taipa, Farah Saleena and Ganjloo, Ali and Md. Salleh, Liza and Selamat, Jinap and Hamid, Azizah and Zaidul, I. S. M. (2011) Comparison of different extraction methods for the extraction of major bioactive flavonoid compounds from spearmint (Mentha spicata L.) leaves. Food and Bioproducts Processing, 89 (1). 67 - 72. ISSN 0960-3085

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Official URL: http://dx.doi.org/10.1016/j.fbp.2010.03.002

Abstract

Different bioactive flavonoid compounds including catechin, epicatechin, rutin, myricetin, luteolin, apigenin and naringenin were obtained from spearmint (MenthaspicataL.) leaves by using conventional soxhlet extraction (CSE) and supercritical carbon dioxide (SC-CO2) extraction at different extraction schemes and parameters. The effect of different parameters such as temperature (40, 50 and 60 °C), pressure (100, 200 and 300 bar) and dynamic extraction time (30, 60 and 90 min) on the super critical carbon dioxide (SC-CO2) extraction of spearmint flavonoids was investigated using full factorial arrangement in a completely randomized design (CRD). The extracts of spearmint leaves obtained by CSE and optimal SC-CO2 extraction conditions were further analyzed by high performance liquid chromatography (HPLC) to identify and quantify major bioactive flavonoid compounds profile. Comparable results were obtained by optimum SC-CO2 extraction condition (60 °C, 200 bar, 60 min) and 70% ethanol soxhlet extraction. As revealed by the results, soxhlet extraction had a higher crude extract yield (257.67 mg/g) comparing to the SC-CO2extraction (60.57 mg/g). Supercritical carbon dioxide extract (optimum condition) was found to have more main flavonoid compounds (seven bioactiveflavonoids) with high concentration comparing to the 70% ethanol soxhlet extraction (five bioactiveflavonoids). Therefore, SC-CO2extraction is considered as an alternative process compared to the CSE for obtaining the bioactiveflavonoidcompounds with high concentration from spearmint leaves.

Item Type:Article
Uncontrolled Keywords:spearmint (MenthaspicataL.), bioactiveflavonoid, supercritical carbon dioxide (SC-CO2) extraction, conventional soxhlet extraction (CSE), high performance liquid chromatography (HPLC)
Subjects:Unspecified
Divisions:Chemical Engineering
ID Code:22965
Deposited By: Kamariah Mohamed Jong
Deposited On:27 Nov 2012 01:48
Last Modified:27 Nov 2012 01:53

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