Universiti Teknologi Malaysia Institutional Repository

Biodegradation of 4-aminobenzenesulfonate by Ralstonia sp. PBA and Hydrogenophaga sp. PBC isolated from textile wastewater treatment plant

Gan, H. M. and Shahir, Shafinaz and Ibrahim, Zaharah and Yahya, Adibah (2010) Biodegradation of 4-aminobenzenesulfonate by Ralstonia sp. PBA and Hydrogenophaga sp. PBC isolated from textile wastewater treatment plant. Chemosphere, 82 (4). 507 - 513. ISSN 0045-6535

[img] PDF (Abstract)
77Kb

Official URL: http://dx.doi.org/10.1016/j.chemosphere.2010.10.09...

Abstract

A co-culture consisting of Hydrogenophaga sp. PBC and Ralstonia sp. PBA, isolated from textile wastewater treatment plant could tolerate up to 100mM 4-aminobenzenesulfonate (4-ABS) and utilize it as sole carbon, nitrogen and sulfur source under aerobic condition. The biodegradation of 4-ABS resulted in the release of nitrogen and sulfur in the form of ammonium and sulfate respectively. Ninety-eight percent removal of chemical oxygen demand attributed to 20mM of 4-ABS in cell-free supernatant could be achieved after 118h. Effective biodegradation of 4-ABS occurred at pH ranging from 6 to 8. During batch culture with 4-ABS as sole carbon and nitrogen source, the ratio of strain PBA to PBC was dynamic and a critical concentration of strain PBA has to be reached in order to enable effective biodegradation of 4-ABS. Haldane inhibition model was used to fit the degradation rate at different initial concentrations and the parameters µmax, Ks and Ki were determined to be 0.13h-1, 1.3mM and 42mM respectively. HPLC analyses revealed traced accumulation of 4-sulfocatechol and at least four unidentified metabolites during biodegradation. This is the first study to report on the characterization of 4-ABS-degrading bacterial consortium that was isolated from textile wastewater treatment plant.

Item Type:Article
Uncontrolled Keywords:16S rDNA, 4-aminobenzenesulfonate, biodegradation, hydrogenophaga, ralstonia
Subjects:Q Science > Q Science (General)
Divisions:Biosciences and Bioengineering (Formerly known)
ID Code:22880
Deposited By: Kamariah Mohamed Jong
Deposited On:28 Nov 2012 00:32
Last Modified:28 Nov 2012 00:35

Repository Staff Only: item control page