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Production of cyclodextrin glucanotransferase from alkalophilic Bacillus sp. TS1-1: Optimization of carbon and nitrogen concentration in the feed medium using central composite design

Wan Md. Zain, Wan Salwanis and Illias, Rosli and Md Salleh, Madihah and Hassan, Osman and Abdul Rahman, Roshanida and Abdul Hamid, Aidil (2007) Production of cyclodextrin glucanotransferase from alkalophilic Bacillus sp. TS1-1: Optimization of carbon and nitrogen concentration in the feed medium using central composite design. Biochemical Engineering Journal, 33 . pp. 26-33. ISSN 1369-703X

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Official URL: http://dx.doi.org/10.1016/j.bej.2006.09.024

Abstract

Optimisation of nutrient feeding was developed to overcome the limitation in batch fermentation and to increase the CGTase production from Bacillus sp. TS1-1 in fed batch fermentation. Optimisation of the C/N ratio in the feed stream was conducted in a 5 l fermenter, where feeding was initiated at constant rate of 0.02 h−1. In our initial screening process, the addition of nitrogen source boosted the growth of the microbes, but on the other hand reduced the CGTase production. The amount of tapioca starch and yeast extract was optimised in order to obtain a sufficient growth and thus, increased the CGTase production. Results were analysed using three-dimensional response surface plot, and the optimised values of carbon and nitrogen concentration of 3.30% (w/v) and 0.13% (w/v) were obtained, respectively. CGTase activity increased up to 80.12 U/ml, which is 13.94% higher as compared to batch fermentation (70.32 U/ml). This also led to 14.54% increment of CGTase production in fed batch culture as compared to the production before the optimisation. The CGTase activity obtained was close to the predicted value, which is 78.05 U/ml. © 2006 Elsevier B.V. All rights reserved. Keywords: Cyclodextrin glucanotransferase; C/N ratio; Fed batch; Central composite design

Item Type:Article
Uncontrolled Keywords:Cyclodextrin glucanotransferase, C/N ratio, fed batch, central composite design
Subjects:T Technology > TP Chemical technology
Divisions:Science
ID Code:219
Deposited By: Prof Dr. Noor Aini Abdul Rashid
Deposited On:15 Feb 2007 02:53
Last Modified:17 Feb 2012 05:20

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