Lee, T. H. (2005) Preliminary study of bird's nest recovery process. In: Malaysian Science and Technology Congress 2008, 2005, Kuala Lumpur.
Bird’s nest is a Chinese delicacy that has been claimed to contain a number of health promoting qualities including its high value of protein. The conventional method in cleaning the bird’s nest is laborious ant time consuming. In this study, a novel method based on a series of downstream processes was studied to simplify the cleaning process. Two types of bird nests were investigated, the house nest and the cave nest. The bird’s nest was dissolved in NaOH solution where the insoluble impurities were discarded via centrifugation. The dissolved protein was then recovered based on isoelectric precipitation using HCl solution, centrifugation and freeze drying (-50’C, 5h). Product yield was calculated based on the amount of protein recovered following freeze drying as compared to the protein in the crude bird’s nest. The major scopes were to investigate the effects of NaOH concentrations on protein dissolution, to optimize the product yield during the precipitation process and to analyze the content of the bird’s nest. The best concentration of NaOH for dissolving the cave nest and house nest was 0.38M and 0.32M respectively in this study. The time required for complete dissolution at the optimum NaOH concentration was found to be 24 h and 48 h for the house nest and cave nest respectively. Majority of the cave nest’s protein was found to be precipitated at pH 3.2 while for house nest the value was pH 3.6. Proximate analysis showed that protein in recovered house nest and cave nest was 3.18% and 23.86% respectively higher than that in crude bird’s nest.
|Item Type:||Conference or Workshop Item (Paper)|
|Uncontrolled Keywords:||bird’s nest, protein, alkaline dissolution, acid precipitation|
|Subjects:||Q Science > QD Chemistry|
|Divisions:||Chemical and Natural Resources Engineering (Formerly known)|
|Deposited By:||Liza Porijo|
|Last Modified:||19 Jan 2012 08:51|
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