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A Potential Use Of Dehalogenase D (Dehd) From Rhizobium Sp. For Industrial Process

Huyop, Fahrul Z. and Cooper, Ronald A. (2003) A Potential Use Of Dehalogenase D (Dehd) From Rhizobium Sp. For Industrial Process. Jurnal Teknologi C (39C). pp. 1-8. ISSN 0127-9696

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Official URL: http://www.penerbit.utm.my/onlinejournal/39/C/JTKK...

Abstract

The Rhizobium sp. DehL and DehD were produced by heterologous expression of the cloned gene in E.coli and both proteins were purified using anion-exchange column chromatography. DehL and DehD were characterised by kinetic analysis to determine their Km, Kcat and the Specificity constant. The kinetic analysis showed that DehD from Rhizobium sp. has lower Km value (0.04 mM with D,L-2-CP), higher Kcat (6.28 sec–1 for D,L-2-CP) and Specificity constant (1.46 ×105 M–1sec–1 for D,L-2-CP) compared to other D-specific dehalogenases from different organism suggesting DehD enzyme from Rhizobium sp. is a better catalyst. Km, Kcat and Specificity constant for DehL were 0.12 mM, 25 s–1 and 2.08 × 105 M–1s–1, respectively, using D,L-2CP as the substrate. D-2-haloacid dehalogenase is important for industrial biocatalysis compared to the L-2-haloacid and the kinetic data of DehD hold promise for further development to be used in an industrial process.

Item Type:Article
Uncontrolled Keywords:Dehalogenase, DehL, DehD, Rhizobium
Subjects:Q Science > QH Natural history > QH301 Biology
Divisions:Science
ID Code:2062
Deposited By: Pn Norhayati Abu Ruddin
Deposited On:22 Mar 2007 00:52
Last Modified:21 Sep 2010 08:25

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