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Harnessing electro driven separation technique for the separation of selected agrochemicals

Wan Ibrahim, Wan Aini and Monjurul Alam, S. M. and Sulaiman, Azli (2008) Harnessing electro driven separation technique for the separation of selected agrochemicals. The Malaysian Journal of Analytical Sciences, 12 (1). 39 - 45. ISSN 1394-2506

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Official URL: http://www.ukm.my/mjas/v12_n1/7.pdf


Electro driven separation techniques offer a different approach to the analysis of complex mixtures than do traditional pressure-driven chromatographic system; it may rely on electrophoresis, the transport of charged species through a medium by an applied field or may rely on electro driven mobile phase to provide a true chromatographic separation. In the current work the potential of an electro driven separation technique viz. micellar electrokinetic chromatography (MEKC), is harnessed for the separation of selected agrochemicals (organophosphorus pesticides, OPPs) widely used in the agriculture sector in Malaysia. The current study compares the use of MEKC in normal mode (NM) and reverse mode (RM) for the separation of the selected OPPS. This study also highlights the difference in separations produced by performing separations in normal mode-MEKC (NM-MEKC) and reverse mode-MEKC (RM-MEKC) for the selected OPPs. In RM-MEKC, separation is conducted at acidic pH (pH 2.5 in the current work) where the electroosmotic flow (EOF) is weak whereas in NM-MEKC, the separation is carried out under basic pH (9.3 in this work) where the EOF is strong. A reverse migration order of the OPPs was observed under RM-MEKC. Separation under NM-MEKC was found to be superior to those of RMMEKC. A comparison is also made between separations performed under sweeping-NM-MEKC and sweeping-RM-MEKC. In sweeping, the OPPs are prepared in the same background solution (BGS) minus the micelles and is adjusted to the same conductivity as the BGS. The study showed that NM-MEKC is more sensitive than RM-MEKC but sweeping-RM-MEKC is superior to sweeping-NM-MEKC. However, sweeping-RM-MEKC only separates two of the OPPs in a single run whereas sweeping-NM-MEKC separates four OPPs in a single run. The better choice of separation mode would be sweeping-NMMEKC for more OPPs separation in a single run.

Item Type:Article
Uncontrolled Keywords:Micellar electrokinetic chromatography (MEKC), organophosphorus pesticides, normal mode MEKC, reverse mode MEKC, sweeping
Subjects:Q Science > QD Chemistry
ID Code:18879
Deposited By: Kamariah Mohamed Jong
Deposited On:19 Nov 2012 06:31
Last Modified:19 Nov 2012 06:31

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