Isolation, purification and screening of fingal strain for effective bioconversion of palm oil mlll effluent

Abstract

A study was conducted to evaluate potential fungal strains isolated from palm oil effluent (POME) sludge Dengkil, Kuala Lumpur Malaysia. A total of 20 fungal strains were isolated for the production of lignocellulolytic enzyme. The isolated strains were purified by conventional techniques and identified by microscopic examination. The strains isolated belong to the genera of Penicillum. Six strains were selected for the screening test using POME as media for the production of lignocellulolytic enzyme. During the screening, three parameters were measured to evaluate the potential fungal strain for the production of cellulase and lignin peroxidase (LiP). The parameters were; pH, total suspended solids (TSS) and chemical oxygen demand (COD) were analysed during this study to get the maximum production of lignocellulolytic enzyme. The cellulase production of strain P1-EFB yield 33 filter paper units (FPU/ml) compared to other tested strains, while lignin peroxidase produced 5038 unit enzyme per litre (U/L) of POME sludge. The COD removal was observed in the treatment sludge to be 95% by this strain.