Universiti Teknologi Malaysia Institutional Repository

The performance of different pre-treatment techniques in the isolation and purification of monoclonal IgG antibody

Loh, Melissa Pei Shian and Abdul Majid, Fadzilah Adibah (2006) The performance of different pre-treatment techniques in the isolation and purification of monoclonal IgG antibody. In: 1st International Conference on Natural Resources Engineering & Technology 2006, 24-25th July 2006, Putrajaya, Malaysia.

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Abstract

In this study, the effects of different sample preparation techniques on the separation of monoclonal antibody IgG1 were investigated experimentally. Monoclonal IgG1 was obtained from hybridoma cell line TB/C3 transfected with bcl-2 carrier plasmid, which was grown in serum-free medium. Three different pre-treatment techniques prior to Protein G affinity chromatography have been used in order to concentrate and partial purify the monoclonal antibody. The pre-treatments researched in this paper are precipitation of the antibody by ammonium sulfate, dilution of the antibody in the binding buffer of affinity chromatography and ultrafiltration through an Amicon Ultra-15 filter with molecular weight cut-off at 100 kDa. Purification through direct application of the antibody onto the Protein G affinity column without pre-treatments was used as a control method. The results indicate that the ultrafiltration through an Amicon filter was an effective method for both concentration and partial purification of the antibodies in laboratory scale.

Item Type:Conference or Workshop Item (Paper)
Uncontrolled Keywords:Pre-treatment; IgG; Protein G; affinity chromatography
Subjects:Q Science > QR Microbiology
Divisions:Chemical and Natural Resources Engineering (Formerly known)
ID Code:178
Deposited By: Cik Rasyidah Mohd Daud
Deposited On:15 Feb 2007 11:38
Last Modified:01 Jun 2010 02:41

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