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Partial characterization of thermostable pullulanase from bacillus flavothermus Kwf-1

Abu Samah, Rozaimi and Md. Salleh, Madihah and Hassan, Osman and Md. Illias, Rosli (2005) Partial characterization of thermostable pullulanase from bacillus flavothermus Kwf-1. Proc. KUSTEM 4th Annual Seminar 2005 . pp. 960-963.

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Abstract

Pullulanase is a debranching enzyme that hydrolyses α-1,6-linkages in starch, amylopectin, pullulan and related oligosaccharides. Thermostable pullulanase is being given a special interest in improving the starch conversion process. A thermophilic bacterial strain producing pullulanase enzyme was isolated from local hot spring. The strain was identified as Bacillus flavothermus KWF-1. In the absence of substrate, the crude enzyme was stable in the range of pH 7-10 and 60-90°C. The optimum pH and temperature for the crude enzyme activity were 10 and 80°C, respectively. For pullulanase production, tapioca starch was found to be the best carbon source.

Item Type:Article
Subjects:Q Science > Q Science (General)
Divisions:Science
ID Code:1762
Deposited By: Dr. Adibah Yahya
Deposited On:20 Mar 2007 00:45
Last Modified:18 Jan 2012 07:24

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