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Transient gene expression of impatiens balsamina axillary meristem by biolistic method

Wagiran, Alina and Andrew, Wetten and Nicholas Hugh, Battey Transient gene expression of impatiens balsamina axillary meristem by biolistic method. Proc. KUSTEM 4th Annual Seminar 2005. . pp. 567-577.

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Abstract

A plasmid-mediated transformation method was developed utilizing the axillary meristem of cotyledon explants of Impatiens balsamina var Dwarf Bush. Low transient transformation frequencies were improved by a series of physical parameters such as helium pressure, target distance, target radius, explants orientation and pDNA concentration as biological parameter. In order to maximize axillary meristem of cotyledon sections saturated with microcarriers, a novel overlapping orientation of explants was utilized. In this way, a transient transformation frequency of 50% was obtained and 2.5% of stable gene expression was achieved after 5 weeks on selective media.

Item Type:Article
Uncontrolled Keywords:Biolistic, Impatiens balsamina, Axillary meristem, cotyledon, GUS
Subjects:Q Science > Q Science (General)
ID Code:1748
Deposited By: Dr. Adibah Yahya
Deposited On:20 Mar 2007 00:48
Last Modified:18 Jan 2012 06:28

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