Scale-Up Of Stirred And Aerated BioengineeringTM Bioreactor Based On Constant Mass Transfer Coefficient

Abstract

The Scale-Up Studies Based On The Constant Oxygen Transfer Coefficient, Kla From 16 L To 150 L Of Aerated And Agitated Bioreactor Were Performed. By Employing The Static Gassing Out Techniques, The Kla Values Were Calculated At Different Sets Of Impeller Speeds And Air Flow Rates Performed In Various Viscosities And Temperatures In The 16 L And 150 L Bioengineeringtm Stirred Bioreactor. Empirical Correlation Was Employed To Correlate And Investigate The Dependence Of Kla On Specific Power Input And Superficial Air Velocity. In Maintaining A Constant Kla Upon Scale-Up From 16 L To 150 L, The Specific Power Input And The Superficial Air Velocity Cannot Be Maintained, Thus An Adjustment Has To Be Done. From The Experimental Results At 150 L, It Was Discovered That The Specific Power Input From 0.0001 To 4.2 Kw/M3 And Superficial Air Velocity Within The Range Of 9 × 10-4 M/S To 7 × 10-3 M/S Employed Gave A Comparable Kla Values Achieved At 16 L Scale. Hence, The Calculated Scaling-Up Factor For Impeller Speed And Air Flow Rate Were 0.28 And 3.1, Respectively. The Comparable Results Obtained From Both Scales Confirmed That The Scale-Up Protocol Developed Works.