Universiti Teknologi Malaysia Institutional Repository

Single cell injection using nano pipette via nanorobotic manipulation system inside E-SEM

Shen, Yajing and Nakajima, Masahiro and Ahmad, Mohd. Ridzuan and Fukuda, Toshio and Kojima, Seji and Homma, Michio (2009) Single cell injection using nano pipette via nanorobotic manipulation system inside E-SEM. In: Proceedings of the 9th IEEE Conference on Nanotechnology (IEEE NANO 2009), 2009, Genoa, Italy.



In this work, a nano injection system for single cell with small size (diameter: ~5 �¿m) is proposed. An environmental scanning electron microscope (E-SEM) nanorobotic manipulation system has been constructed with three units and seven degrees of freedom (DOFs) in total. It can provide a real-time observation with nanoscale resolution during the experiment. Micro pulling and FIB etching are used to control the tip size of nano pipette accurately. In situ penetration of single wild-type yeast cell (W303) is performed using nano pipette inside the E-SEM based on the nanorobotic manipulation system. Spouting experiment of Rhodamine 6G fluorescent solutions at femtoliter level from nano pipette is also achieved under the E-SEM environment. The results show that it is possible to perform small single cell injection using nano pipettes inside the E-SEM.

Item Type:Conference or Workshop Item (Paper)
Uncontrolled Keywords:environmental-SEM, nano pipette injection, nanomanipulation, single cell analysis, yeast cells
Subjects:T Technology > TK Electrical engineering. Electronics Nuclear engineering
Divisions:Electrical Engineering
ID Code:15744
Deposited By: Mrs Liza Porijo
Deposited On:07 Oct 2011 10:01
Last Modified:07 Oct 2011 10:01

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