Shen, Yajing and Nakajima, Masahiro and Ahmad, Mohd. Ridzuan and Fukuda, Toshio and Kojima, Seji and Homma, Michio (2009) Single cell injection using nano pipette via nanorobotic manipulation system inside E-SEM. In: Proceedings of the 9th IEEE Conference on Nanotechnology (IEEE NANO 2009), 2009, Genoa, Italy.
In this work, a nano injection system for single cell with small size (diameter: ~5 Ã?Â¿m) is proposed. An environmental scanning electron microscope (E-SEM) nanorobotic manipulation system has been constructed with three units and seven degrees of freedom (DOFs) in total. It can provide a real-time observation with nanoscale resolution during the experiment. Micro pulling and FIB etching are used to control the tip size of nano pipette accurately. In situ penetration of single wild-type yeast cell (W303) is performed using nano pipette inside the E-SEM based on the nanorobotic manipulation system. Spouting experiment of Rhodamine 6G fluorescent solutions at femtoliter level from nano pipette is also achieved under the E-SEM environment. The results show that it is possible to perform small single cell injection using nano pipettes inside the E-SEM.
|Item Type:||Conference or Workshop Item (Paper)|
|Uncontrolled Keywords:||environmental-SEM, nano pipette injection, nanomanipulation, single cell analysis, yeast cells|
|Subjects:||T Technology > TK Electrical engineering. Electronics Nuclear engineering|
|Deposited By:||Liza Porijo|
|Deposited On:||07 Oct 2011 10:01|
|Last Modified:||07 Oct 2011 10:01|
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