Salmiati, Salmiati and M. R., Salim and Ujang, Z. and Olsson, G. (2009) Recovery of polyhydroxyalkanoates (PHAs) from mixed microbial cultures by simple digestion and saponification. In: Proceedings of the 3rd International Water Association (IWA)-ASPIRE Conference and Exhibition,, 2009, Taiwan.
Highly efficient separation and purification of polyhydroxyalkanoates (PHAs) from PHA-containing cell mass is essential for the production of bioplastics from renewable resources in a cost-effective, environmentally friendly way. Combination of digestion and selective dissolution of non-PHA cell mass (NPCM) using low concentration of sodium hypochlorite of PHA biopolymers, a simple process is developed and has demonstrated to recover PHA from cell mass to high purity (>90 wt %) with high yield (>90 wt %). Saponification was applied to separate or remove oil from the PHA-particles. This method was adopted in this study and was able to increase the PHA extraction from the mixed microbial intracellular biomass up to 85% of cell dried weight (CDW).
|Item Type:||Conference or Workshop Item (Paper)|
|Uncontrolled Keywords:||recovery, digestion, PHAs, purification, inclusion body, saponification|
|Subjects:||T Technology > TA Engineering (General). Civil engineering (General)|
|Deposited By:||Liza Porijo|
|Deposited On:||05 Oct 2011 09:06|
|Last Modified:||05 Oct 2011 09:06|
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