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Recovery of polyhydroxyalkanoates (PHAs) from mixed microbial cultures by simple digestion and saponification

Salmiati, Salmiati and M. R., Salim and Ujang, Z. and Olsson, G. (2009) Recovery of polyhydroxyalkanoates (PHAs) from mixed microbial cultures by simple digestion and saponification. In: Proceedings of the 3rd International Water Association (IWA)-ASPIRE Conference and Exhibition,, 2009, Taiwan.

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Abstract

Highly efficient separation and purification of polyhydroxyalkanoates (PHAs) from PHA-containing cell mass is essential for the production of bioplastics from renewable resources in a cost-effective, environmentally friendly way. Combination of digestion and selective dissolution of non-PHA cell mass (NPCM) using low concentration of sodium hypochlorite of PHA biopolymers, a simple process is developed and has demonstrated to recover PHA from cell mass to high purity (>90 wt %) with high yield (>90 wt %). Saponification was applied to separate or remove oil from the PHA-particles. This method was adopted in this study and was able to increase the PHA extraction from the mixed microbial intracellular biomass up to 85% of cell dried weight (CDW).

Item Type:Conference or Workshop Item (Paper)
Uncontrolled Keywords:recovery, digestion, PHAs, purification, inclusion body, saponification
Subjects:T Technology > TA Engineering (General). Civil engineering (General)
Divisions:Others
ID Code:15653
Deposited By: Liza Porijo
Deposited On:05 Oct 2011 09:06
Last Modified:05 Oct 2011 09:06

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