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Fosmid-based library of alkaliphilic bacillus sp. G1

Md. Illias, Rosli and Muhammad Noor, Yusuf (2009) Fosmid-based library of alkaliphilic bacillus sp. G1. In: 2nd International Conference & Workshps on Basic & Applied Sciences & Regional Annual Fundamental Science Seminar 2009 (ICORAFFS 2009), 2009, The Zon Regency Hotel, Johor.

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Abstract

Previous studies isolated a local alkaliphilic Bacillus species, designated Bacillus sp. G1. The GSS (Genome Sequencing Survey) done then discovered that the alkaliphile also contained genes encoding for antiporter proteins and various enzymes such as proteases and glucanases, among others. Currently an effort is in progress to sequence the whole genome of the organism, so as to fully exploit the vast potential of the alkaliphilic genes and alkalitolerant mechanisms contained within it. For this purpose, the bulk sequence data was separately obtained via 454’s pyrosequencing and organized into many separate contigs and scaffolds. A fosmid library was then constructed with the goal of assembling the detached data into a single complete genome sequence. Approximately 8000 fosmid library clones with insert sizes ranging from 20-50kb were produced, and confirmation of inserts were done via representative fosmid extraction and subsequently restriction digestion.

Item Type:Conference or Workshop Item (Paper)
Uncontrolled Keywords:alkaliphile, bacillus sp. G1, fosmid-based library, genome sequencing
Subjects:Q Science > QD Chemistry
Divisions:Chemical and Natural Resources Engineering (Formerly known)
ID Code:15209
Deposited By: Liza Porijo
Deposited On:21 Sep 2011 09:57
Last Modified:21 Sep 2011 10:03

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