Watanabe, Yuta and Ikegami, Tohru and Horie, Kanta and Hara, Takeshi and Jaafar, Jafariah and Tanaka, Nobuo (2009) Improvement of separation efficiencies of anion-exchange chromatography using monolithic silica capillary columns modified with polyacrylates and polymethacrylates containing tertiary amino or quaternary ammonium groups. Journal of Chromatography A, 1216 (44). pp. 7394-7401. ISSN 00219673
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Official URL: http://dx.doi.org/10.1016/j.chroma.2009.07.012
Anion-exchange (AEX) columns were prepared by on-column polymerization of acrylates and methacrylates containing tertiary amino or quaternary ammonium groups on monolithic silica in a fused silica capillary modified with anchor groups. The columns provided a plate height (H) of less than 10 µm at optimum linear velocity (u) with keeping their high permeability (K = 9-12 × 10-14 m2). Among seven kinds of AEX columns, a monolithic silica column modified with poly(2-hydroxy-3-(4-methylpiperazin-1-yl)propyl methacrylates) (HMPMA) showed larger retentions and better selectivities for nucleotides and inorganic anions than the others. The HMPMA column of 410 mm length produced 42 000-55 000 theoretical plates (N) at a linear velocity of 0.97 mm/s with a backpressure of 3.8 MPa. The same column could be employed for a fast separation of inorganic anions in 1.8 min at a linear velocity of 5.3 mm/s with a backpressure of 20 MPa. In terms of van Deemter plot and separation impedance, the HMPMA column showed higher performance than a conventional particle-packed AEX column. The HMPMA column showed good recovery of a protein, trypsin inhibitor, and it was applied to the separation of proteins and tryptic digest of bovine serum albumin (BSA) in a gradient elution, to provide better separation compared to a conventional particle-packed AEX column.
|Uncontrolled Keywords:||anion-exchange chromatography, inorganic ions, monolithic silica column, nucleotides|
|Subjects:||Q Science > QD Chemistry|
|Deposited By:||Ms Zalinda Shuratman|
|Deposited On:||30 Sep 2011 15:07|
|Last Modified:||30 Sep 2011 15:07|
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