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Improvement of separation efficiencies of anion-exchange chromatography using monolithic silica capillary columns modified with polyacrylates and polymethacrylates containing tertiary amino or quaternary ammonium groups

Watanabe, Yuta and Ikegami, Tohru and Horie, Kanta and Hara, Takeshi and Jaafar, Jafariah and Tanaka, Nobuo (2009) Improvement of separation efficiencies of anion-exchange chromatography using monolithic silica capillary columns modified with polyacrylates and polymethacrylates containing tertiary amino or quaternary ammonium groups. Journal of Chromatography A, 1216 (44). pp. 7394-7401. ISSN 00219673

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Official URL: http://dx.doi.org/10.1016/j.chroma.2009.07.012

Abstract

Anion-exchange (AEX) columns were prepared by on-column polymerization of acrylates and methacrylates containing tertiary amino or quaternary ammonium groups on monolithic silica in a fused silica capillary modified with anchor groups. The columns provided a plate height (H) of less than 10 µm at optimum linear velocity (u) with keeping their high permeability (K = 9-12 × 10-14 m2). Among seven kinds of AEX columns, a monolithic silica column modified with poly(2-hydroxy-3-(4-methylpiperazin-1-yl)propyl methacrylates) (HMPMA) showed larger retentions and better selectivities for nucleotides and inorganic anions than the others. The HMPMA column of 410 mm length produced 42 000-55 000 theoretical plates (N) at a linear velocity of 0.97 mm/s with a backpressure of 3.8 MPa. The same column could be employed for a fast separation of inorganic anions in 1.8 min at a linear velocity of 5.3 mm/s with a backpressure of 20 MPa. In terms of van Deemter plot and separation impedance, the HMPMA column showed higher performance than a conventional particle-packed AEX column. The HMPMA column showed good recovery of a protein, trypsin inhibitor, and it was applied to the separation of proteins and tryptic digest of bovine serum albumin (BSA) in a gradient elution, to provide better separation compared to a conventional particle-packed AEX column.

Item Type:Article
Uncontrolled Keywords:anion-exchange chromatography, inorganic ions, monolithic silica column, nucleotides
Subjects:Q Science > QD Chemistry
Divisions:Science
ID Code:15110
Deposited By: Ms Zalinda Shuratman
Deposited On:30 Sep 2011 15:07
Last Modified:30 Sep 2011 15:07

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