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Cloning of family 11 Xylanase, xynG1 from locally isolated aspergillus fumigatus RT-1 & its expression in escherichia coli

Md. Illias, Rosli (2009) Cloning of family 11 Xylanase, xynG1 from locally isolated aspergillus fumigatus RT-1 & its expression in escherichia coli. In: 2nd International Conference & Workshops on Basic & Applied Sciences and Regional Annual Fundamental Science Seminar 2009 (ICORAFSS 2009), 2009, The ZON Regency Hotel, Johor.

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Official URL: http://www.ibnusina.utm.my/icorafss/

Abstract

Locally isolated fungi are screened for xylanase activity via Remazol Brilliant Blue-xylan agar plate for hydrolysis of 1,4-beta-D-xylosidic linkages and dinitrosalicylic acid (DNS) method for release of xylose. Two of the fungi which showed the highest xylanase activity is identified as Aspergillus fumigatus RT-1, with activity 8.1 U/ml, followed by Aspergillus fumigatus RT-2, with activity 7.8 U/mL. Family 11 xylanase consensus fragments are amplified from genomic DNA by PCR using consensus degenerate primers that exhibited the conserved motifs within fungal family 11 xylanase genes. Two full-length xylanase genes, named xlnA and xynG1 are obtained by PCR and RT-PCR using specific primers designed based on the sequences in the database. The full-length xlnA contained 739 bp, including one intron of 52 bp while the full-length xynG1 contained 712 bp, with one intron of 46 bp. The mature peptide of xynG1 is cloned into pET-21a and pET-22b and expressed in Escherichia coli BL21 (DE3).

Item Type:Conference or Workshop Item (Paper)
Subjects:Q Science > QD Chemistry
Q Science > QK Botany
Divisions:Chemical and Natural Resources Engineering (Formerly known)
ID Code:14979
Deposited By: Mrs Liza Porijo
Deposited On:19 Sep 2011 09:28
Last Modified:10 Jul 2017 07:39

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