Universiti Teknologi Malaysia Institutional Repository

Characterization and purification of extracellular polymeric substances exhibiting antibacterial activity from marine epibiotic bacteria

Ruslan, Rudzanna and Shamsir, Mohd. Shahir (2009) Characterization and purification of extracellular polymeric substances exhibiting antibacterial activity from marine epibiotic bacteria. In: Second International Conference and Workshops on Basic and Applied Sciences (2nd ICOWOBAS) Regional Annual Fundamental Science Seminar 2009 (RAFSS 2009), , 2009, Johor Bahru, Malaysia.

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Abstract

The aim of this study is to purify and characterize the extracellular polymeric substances (EPS) with antibacterial activity produced by an unknown epibiotic marine bacterium. Extracellular mucus from marine sourced bacterial species has been shown to possess antibacterial compound that is of interest to the pharmaceutical industry. Comparison of extraction and purification method was conducted and results showed that the seawater extraction was determined to be the best method. From the colorimetric analyses, the content of protein, carbohydrate and uronic acid was 0.1040 g/L (12.18 %), 0.6539 g/L (76.59 %) and 0.0959 g/L (11.23 %), respectively. The range of molecular weight of EPS was determined between 109.370 kDa to 20.621 kDa. Anti-microbial activity test were also conducted and showed positive results against eight bacteria; Pseudomonas aeruginosa, Proteus vulgaris, Streptococcus faecalis, Staphylococcus aureus, Bacillus cereus, Enterobacter aerogenes, Escherichia coli, and Micrococcus luteus. A variant of the disk diffusion test with pre-diffusion performed on the agar prior to incubation showed increased anti-microbial inhibition in pre-diffused agar. This study successfully identified the best purification, extraction and testing method for marine sourced microorganism and identified the presence of a potential anti microbial compound.

Item Type:Conference or Workshop Item (Paper)
Subjects:Q Science > Q Science (General)
Divisions:Biosciences and Bioengineering (Formerly known)
ID Code:14898
Deposited By: Liza Porijo
Deposited On:15 Sep 2011 02:44
Last Modified:15 Sep 2011 02:44

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