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Biodegradition of monochloroacetic acid by a presumptive Pseudomonas sp.strain R1 bacterium isolated from Malaysia paddy (rice) field

Ismail, Siti Nurmadihah and Mohd. Taha, Aishah and Jing, Ng Hong and Ab. Wahab, Roswanira and Andul Hamid, Aidil and Pakingking Jr, Rolando V. and Huyop, Fahrul Zaman (2009) Biodegradition of monochloroacetic acid by a presumptive Pseudomonas sp.strain R1 bacterium isolated from Malaysia paddy (rice) field. In: Research Management Centre, 2009, n/a.

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Abstract

A bacterial strain tentatively identified as Pseudomonas sp. R1 was isolated from a paddy (rice) field that could degrade monochloroacetic acid (MCA) for concentrations ranging from 5 to 40 mM. Quantitative agreement between the amount of MCA introduced and chloride released was also found. MCA dehalogenase activity in this strain was found to be inducible. Cell-free extracts displayed dehalogenating activity with specific halogenated organic compound with no activity on dichloropropionic acid or monochloropropionic acid. The estimated Km values for MCA was 0.14 mM. The optimal pH range for MCA dehalogenase activity (between pH 6.5 and 8.0), whereas the thermal stability profile stable up to 50 °C. The results of our current study demonstrated the potential use of Pseudomonas sp. R1 as suitable biological agent for biodegradation of MCA in contaminated agricultural area.

Item Type:Conference or Workshop Item (Paper)
Uncontrolled Keywords:dehalogenase, biodegradation, monochloroacetate, Pseudomonas sp.
Subjects:Q Science > Q Science (General)
Divisions:Science
ID Code:14874
Deposited By: Liza Porijo
Deposited On:15 Sep 2011 02:45
Last Modified:15 Sep 2011 02:45

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