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A further characterization of 3-chloropropionic acid dehalogenase from rhodococcus sp. HJ1

Jing, Ng Hong and Ab. Wahab, Roswanira and Mohd. Taha, Aishah and Abdul Rashid, Noor Aini and Huyop, Fahrul (2009) A further characterization of 3-chloropropionic acid dehalogenase from rhodococcus sp. HJ1. In: International Congress of Malaysian Society for Microbiology (ICMSM 2009), 2009, Park Royal Hotel, Pulau Pinang.

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Official URL: http://dx.doi.org/10.3923/jm.2008.482.488

Abstract

The main aim of the present study is to further characterize a new dehalogenase enzyme found in the crude extracts from Rhodococcus sp. The ability of the enzyme to catalyze the dehalogenation of various halogen-substituted organic acids was investigated and the highest activity was found with 3-chloropropionic acid as a sole carbon source in the growth medium. The enzyme followed Michaelis-Menten kinetics and the Km for 3-chloropropionic acid was 0.2 mM. Maximum activity was found at pH 7.6 at 30°C. The enzyme activity in the cell-free extract was unaffected by diaminoethane tetraacetic acid (EDTA), dithiothreitol (DTT) or by Mn and Zn ions but was reduced by HgCl2 (70%) and Pb(NO3)2 (80%). The enzyme removed the chlorine atom present on a number of 3- and 4-carbon alkanoic acids if the halogen was on the β-position.

Item Type:Conference or Workshop Item (Paper)
Subjects:Q Science > Q Science (General)
T Technology > TA Engineering (General). Civil engineering (General)
Divisions:Biosciences and Bioengineering (Formerly known)
ID Code:14665
Deposited By: Liza Porijo
Deposited On:12 Sep 2011 02:12
Last Modified:12 Sep 2011 02:12

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