Isolation, screening and identification of newly isolated soil streptomyces (streptomyces sp. Nrc-35) for ß-lactamase inhibitor production

Abstract

During the screening study, a total of 60 different Streptomyces species were isolated from soil samples collected from different areas in Egypt. Streptomyces sp. NRC-35was selected for its high ß-lactamase inhibitor activity mediated by the production of clavulanic acid. This newly isolated strain was able to produce up to 65 mg L-1 clavulanic acid. The taxonomical properties of the strain were examined according to International Streptomyces Project (ISP) scheme. The primary identification of this isolate was based on spore morphology and cell wall chemo-type. Furthermore, culture physiological characteristics of the isolated strain, Str. NRC-35 were compared to other reference strains belong to Streptomyces species. The analysis of nucleotide sequence of the 16s Rrna indicated similiarity binary level of 91% with Streptomyces species.