Mesri, Suhailysa and Ab. Wahab, Roswanira and Huyop, Fahrul (2009) Degradation of 3-chloropropionic acid (3CP) by pseudomonas sp B6P isolated from a rice paddy field. Annals of Microbiology, 59 (3). pp. 447-451. ISSN 1590-4261
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Official URL: http://dx.doi.org/10.1007/BF03175129
A bacterium that degrades 3-chloropropionic acid (3CP) was isolated from local paddy (rice) agricultural soil by enrichment culture. Taxonomic analysis identified the strain as Pseudomonas sp. Strain-mediated degradation of 3CP released chloride ions into the growth medium, and the degradation of similar to 100% of 20 mM 3CP was monitored by high performance liquid chromatography (HPLC). The putative Pseudomonas sp. dehalogenase that released the chloride ions from 3CP was induced by growth of the bacterium in the presence of 3CP as the sole carbon source. The dehalogenase showed activity against a specific type of halogenated aliphatic acids, and the highest activity was obtained with 3CP. However, the enzyme could only remove halogen atoms from 3-carbon alkanoic acids if the halogen was at the beta- but not the alpha-position. In crude bacterial extracts, the dehalogenase activity with 3CP was maximum at 30 degrees C, over a broad pH range (pH 7.5 optimum) and had an apparent K(ms) of 0.25 +/- 0.04 mM and 0.67 +/- 0.17 mM for 3CP and 2,3-dichloropropionic acid, respectively.
|Uncontrolled Keywords:||biodegradation, 3-chloropropionic acid, dehalogenase, bioremediation|
|Subjects:||T Technology > TP Chemical technology|
|Divisions:||Biosciences and Bioengineering (Formerly known)|
|Deposited By:||Ms Zalinda Shuratman|
|Deposited On:||03 Aug 2011 07:10|
|Last Modified:||03 Aug 2011 07:10|
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