Ibrahim, Hanan Moawia and Wan Yusoff, Wan Mohtar and Abdul Hamid, Aidil and Md. Illias, Rosli and Hassan, Othman and Omar, Othman (2005) Optimization of medium for the production of ß-cyclodextrin glucanotransferase using central composite design (CCD). Process Biochemistry, 40 (2). pp. 753-758. ISSN 1359-5113
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Official URL: http://dx.doi.org/10.1016/j.procbio.2004.01.042
Abstract
Production of cyclodextrin glucanotransferase (CGTase) from Bacillus G1, a new bacterial isolate was optimized in shake-flask cultures using statistical design approach. An efficient fermentation medium producing CGTase with high activity (54.9 U/ml) was determined. A 25 Central Composite Design (CCD; half fraction) has been chosen to elucidate the combined effect of five process variables; tapioca starch, peptone, yeast extract, magnesium sulphate and potassium phosphate concentration. Concentrations of 4% tapioca starch, 2% peptone, 0.04% magnesium sulphate and 1% sodium carbonate have been shown to be optimum for the production of CGTase.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | cyclodextrin glucanotransferase, CCD, Medium optimization |
| Subjects: | Q Science > QD Chemistry |
| Divisions: | Chemical and Natural Resources Engineering (Formerly known) |
| ID Code: | 13305 |
| Deposited By: | Liza Porijo |
| Deposited On: | 03 Aug 2011 07:11 |
| Last Modified: | 03 Aug 2011 07:11 |
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