Ibrahim, Hanan Moawia and Wan Yusoff, Wan Mohtar and Abdul Hamid, Aidil and Md. Illias, Rosli and Hassan, Othman and Omar, Othman (2005) Optimization of medium for the production of ß-cyclodextrin glucanotransferase using central composite design (CCD). Process Biochemistry, 40 (2). pp. 753-758. ISSN 1359-5113
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Official URL: http://dx.doi.org/10.1016/j.procbio.2004.01.042
Production of cyclodextrin glucanotransferase (CGTase) from Bacillus G1, a new bacterial isolate was optimized in shake-flask cultures using statistical design approach. An efficient fermentation medium producing CGTase with high activity (54.9 U/ml) was determined. A 25 Central Composite Design (CCD; half fraction) has been chosen to elucidate the combined effect of five process variables; tapioca starch, peptone, yeast extract, magnesium sulphate and potassium phosphate concentration. Concentrations of 4% tapioca starch, 2% peptone, 0.04% magnesium sulphate and 1% sodium carbonate have been shown to be optimum for the production of CGTase.
|Uncontrolled Keywords:||cyclodextrin glucanotransferase, CCD, Medium optimization|
|Subjects:||Q Science > QD Chemistry|
|Divisions:||Chemical and Natural Resources Engineering (Formerly known)|
|Deposited By:||Liza Porijo|
|Deposited On:||03 Aug 2011 07:11|
|Last Modified:||03 Aug 2011 07:11|
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