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Optimization of medium for the production of ß-cyclodextrin glucanotransferase using central composite design (CCD)

Ibrahim, Hanan Moawia and Wan Yusoff, Wan Mohtar and Abdul Hamid, Aidil and Md. Illias, Rosli and Hassan, Othman and Omar, Othman (2005) Optimization of medium for the production of ß-cyclodextrin glucanotransferase using central composite design (CCD). Process Biochemistry, 40 (2). pp. 753-758. ISSN 1359-5113

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Official URL: http://dx.doi.org/10.1016/j.procbio.2004.01.042

Abstract

Production of cyclodextrin glucanotransferase (CGTase) from Bacillus G1, a new bacterial isolate was optimized in shake-flask cultures using statistical design approach. An efficient fermentation medium producing CGTase with high activity (54.9 U/ml) was determined. A 25 Central Composite Design (CCD; half fraction) has been chosen to elucidate the combined effect of five process variables; tapioca starch, peptone, yeast extract, magnesium sulphate and potassium phosphate concentration. Concentrations of 4% tapioca starch, 2% peptone, 0.04% magnesium sulphate and 1% sodium carbonate have been shown to be optimum for the production of CGTase.

Item Type:Article
Uncontrolled Keywords:cyclodextrin glucanotransferase, CCD, Medium optimization
Subjects:Q Science > QD Chemistry
Divisions:Chemical and Natural Resources Engineering (Formerly known)
ID Code:13305
Deposited By: Liza Porijo
Deposited On:03 Aug 2011 07:11
Last Modified:03 Aug 2011 07:11

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