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Poly (vinyl Alcohol) : a potential matrix for glucose oxidase immobilization?

A. A., Azila and T., Barbari and P., Searson (2004) Poly (vinyl Alcohol) : a potential matrix for glucose oxidase immobilization? Med Malaysia Jurnal .

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Official URL: http://www.ncbi.nlm.nih.gov/pubmed/15468814

Abstract

Considerable effort has been focused on the method of immobilizing glucose oxidase (GOD) for amperometric glucose biosensors since the technique employed may influence the available activity of the enzyme and thus affect the performance of the sensor. Narrow measuring range and low current response are still considered problems in this area. In this work, poly(vinyl alcohol)(PVA) was investigated as a potential matrix for GOD immobilization. GOD was entrapped in cross-linked PVA. The use of a PVA-GOD membrane as the enzymatic component of a glucose biosensor was found to be promising in both the magnitude of its signal and its relative stability over time. The optimum PVA-GOD membrane (cross-linking density of 0.06) was obtained through careful selection of the cross-linking density of the PVA matrix.

Item Type:Article
Subjects:Q Science > Q Science (General)
T Technology > TA Engineering (General). Civil engineering (General)
Divisions:Biosciences and Bioengineering (Formerly known)
ID Code:12806
Deposited By: S.N.Shahira Dahari
Deposited On:30 Jun 2011 05:34
Last Modified:10 Sep 2012 09:10

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