Isolation and cloning of human NQO1 promoter in pGL3 basic vector

Abstract

Malaria is a major public health problem caused by Plasmodium falciparum, a parasite that infects red blood cells. Recently, several polyphenolic compounds have been reported capable of preventing the progression of malaria parasite. This observation may be related to NAD (P) H: quinon oxidoreductase: a flavoprotein responsible for catalyzing two-electron reduction and detoxification of quinones and their derivatives. In this study the 2123 bp of the 5` upstream of the first transcription start site was successfully isolated. Based on the bioinformatic program, several regulatory regions such as Antioxidant Response Element (ARE) may be responsible for the direct regulation of polyphenols on this enzyme. It was predicted at -477 from the first transcription start site. Upon isolation, this fragment was used in a cloning process into the pGL3 Basic vector and transformed to E.coli DH5? competent cells