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The effects of reaction conditions on the production of gamma-cyclodextrin from tapioca starch by using a novel recombinant engineered CGTase

Goh, K. M. and Mahadi, N. M. and Hassan, Osman and Rahman, R. N. Z. R. A. and Illias, Rosli Md. (2007) The effects of reaction conditions on the production of gamma-cyclodextrin from tapioca starch by using a novel recombinant engineered CGTase. Journal of Molecular Catalysis B - Enzymatic, 49 (1-4). pp. 118-126. ISSN 1381-1177

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Official URL: http://dx.doi.org/10.1016/j.molcatb.2007.09.011

Abstract

A novel mutant enzyme namely H43T CGTase can produce up to 39% gamma-cyclodextrin (gamma-CD) compared to the native enzyme which produces only 10% gamma-CD. The effect of the reaction conditions on gamma-CD production was studied using this mutant CGTase. The effects of substrate-buffer combination, starch pretreatment and concentration, pH, additives and finally the use of a debranching enzyme improved the gamma-CD ratio further. The tapioca-acetate pair gave the highest conversion (16% conversion) among four types of starch and four buffer system combinations. Gelatinized starch was preferred compared to raw tapioca starch in producing a high percentage of gamma-CD and conversion rate. Higher pH especially pH 8 - 9 led to a higher proportion of gamma-CD, and was relatively more apparent when the concentration of starch was increased. Forty-six percent gamma-CD was produced using 2.5% gelatinized tapioca starch at pH 8. Pullulanase enzyme was found to be useful in reducing the viscosity of tapioca starch paste thus increasing the efficiency of utilization of starch by CGTase by at least 20- to 30-fold. Up to 48% gamma-CD can be produced when 4% pullulanase-pretreated tapioca starch was reacted with the CGTase mutant. It was also found that the supplementation of the reaction mixture with glucose. toluene, or cyclododecanone improved the gamma-CD yield by 42.2, 46.4,43.4, and 43.4%, respectively. All the parameters involved have been shown to affect the product specificity of the mutant H43T CGTase transglycosylation mechanism. (c) 2007 Elsevier B.V. All rights reserved.

Item Type:Article
Uncontrolled Keywords:Bacillus sp, G1, cyclodextrin glucanotransferase, ganima-cyclodextrin, pullulanase, site-directed mutagenesis, tapioca starch
Subjects:Q Science > QD Chemistry
Q Science > QH Natural history > QH301 Biology
Divisions:Biosciences and Bioengineering (Formerly known)
ID Code:11709
Deposited By: Nor Asmida Abdullah
Deposited On:14 Jan 2011 01:43
Last Modified:17 Jan 2011 09:28

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