Tapered angle microfluidic device for cell separation using hydrodynamic principle

Abstract

Metastasis is responsible for 90% of all cancer-related fatalities. CTCs are difficult to detect due to their rarity. Currently, the devices that aid in the detection of CTCs have limitations, such as a high price, complex apparatus, or low sample purity. Consequently, the purpose of this research is to construct and enhance a microfluidic device with a tapered angle. This device aims to outperform conventional microfluidic devices in terms of cost, sample purity, and apparatus. Using the finite element simulation software COMSOL Multiphysics, two studies are conducted, the first of which examines the effect of taper angle on particle separation and the second of which examines the effect of flow rate on particle separation. This design enables particle separation based on hydrodynamic theory and the sedimentation process. A mixture of 3 μm and 10 μm polystyrene (PS) microbeads were effectively separated when the taper angle approached 20 degrees, and separation continued until the taper angle reached 89 degrees. Using 12° and 6° taper angles, 3 μm, and 10 μm polystyrene microbeads were not effectively separated using finite element simulation. The proposed product is functionally enticing despite the fact that this design utilizes a passive separation technique. This technology provides uncomplicated, label-free, continuous separation of numerous particles in a self-contained device without the need for cumbersome equipment. Consequently, both point-of-care diagnostic instruments and micro total analytic systems could utilize this device.