Juliansyah, Dandy Akbar and Nurjayadi, Muktiningsih and Declan, Jefferson Lynford and Putri, Gladys Indira and Krisdawati, Ismaya and Azzahra, Maharanianska and Maulana, Irvan and Saamia, Vira and Saputro, Dwi Anna Oktaviani and Wiranatha, I Made and El-Enshasy, Hesham Ali (2023) The potential of grxB gene for detection of C. sakazakii in infant formula milk using real-time polymerase chain reaction. In: 1st International Conference on Sciences, Mathematics, and Education, ICoSMEd 2022, 13 October 2022 - 14 October 2022, Gorontalo, Sulawesi, Indonesia.
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Official URL: http://dx.doi.org/10.1051/e3sconf/202340004010
Abstract
Cronobacter sakazakii is one of the bacteria that causes food poisoning that contaminates infant formula. This pathogen causes necrotizing enterocolitis, sepsis, and meningitis in infants or neonates with reported case fatality rates ranging from 40% to 80%. Therefore, it is necessary to develop fast and accurate detection of C. sakazakii in infant formula milk. This research aims to develop a method for detecting C. sakazakii bacteria using real-time PCR with high sensitivity, specificity, and accuracy. A rapid detection method using real-time PCR with the target gene grxB successfully detects the presence of C. sakazakii DNA in artificially contaminated formula milk. The results of the real-time PCR test showed that C. sakazakii DNA with a concentration of 53 ng/μL could be amplified by the grxB gene primer pair with a Ct value of 12 and a Tm value of 85.8oC. The specificity test showed that the grxB primer could differentiate between target and some non-target bacteria. The sensitivity test showed the ability of the grxB primer to detect the smallest concentration of 3,392 pg/μL with a Ct of 24,06. Based on the results obtained, it can be concluded that the grxB primer has the potential to be used as rapid detection method for C. sakazakii bacteria in infant formula using real-time PCR.
Item Type: | Conference or Workshop Item (Paper) |
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Uncontrolled Keywords: | Cronobacter sakazakii, pathogen, bacteria. |
Subjects: | T Technology > TP Chemical technology |
Divisions: | Chemical and Energy Engineering |
ID Code: | 107804 |
Deposited By: | Muhamad Idham Sulong |
Deposited On: | 02 Oct 2024 07:39 |
Last Modified: | 02 Oct 2024 07:39 |
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