Universiti Teknologi Malaysia Institutional Repository

Phytochemical profiling and pharmaceutical properties of Moringa oleifera leaves powder and seed oil against Hepatocellular carcinoma

Hendra Susanto, Hendra Susanto and Surjani Wonorahardjo, Surjani Wonorahardjo and Wira Eka Putra, Wira Eka Putra and Ahmad Taufiq, Ahmad Taufiq and Sunaryono, Sunaryono and Dianvita Nur Fadhilah, Dianvita Nur Fadhilah and Siti Bachrotus Recha Nur Fa’ida, Siti Bachrotus Recha Nur Fa’ida and Sa’diyatul Rizqie Amaliyah Firdaus, Sa’diyatul Rizqie Amaliyah Firdaus and Moch. Sholeh, Moch. Sholeh and Nik Malek, Nik Ahmad Nizam (2023) Phytochemical profiling and pharmaceutical properties of Moringa oleifera leaves powder and seed oil against Hepatocellular carcinoma. Malaysian Journal of Fundamental and Applied Sciences, 19 (4). pp. 691-706. ISSN 2289-599X

[img] PDF
575kB

Official URL: http://dx.doi.org/10.11113/mjfas.v19n4.2818

Abstract

Hepatocellular carcinoma (HCC) is one of the deadliest types of cancer with a mortality rate of 8.9% of the total cancer deaths in Indonesia. This cancer can be caused by exposure to hepatitis B and C viruses, NAFLD, autoimmune, diabetes to sporadic genetic diseases. The development of chronic HCC is generally preceded by the occurrence of severe liver fibrosis and cirrhosis. One of the genes that play a role in fibrosis in the incidence of HCC is TGF-β1. As a pro-fibrotic cytokine, the presence of high levels of TGF-β1 may be due to oxidative stress activity early in cancer development. One of the natural ingredients with lots of phytochemical content in the form of antioxidants that can reduce this activity is Moringa plant (Moringa oleifera). In this study we used a computational approach using molecular docking on the results of the GC-MS and LC-HRMS tests on Moringa oleifera Seed Oil (MOSEIL) and Moringa oleifera Leaves Powder (MOLP) which are oil and flour products made from moringa. The results of the identification of phytochemical compounds through the GC-MS test showed that the dominant compound in MOSEIL was oleic acid (37.546%) and in MOLP was ester (8.802%) when using n-hexane as solvent. The percentage yield of the dominant compound from the LC-HRMS test in MOSEIL was nitro compound (72.55%) and at MOLP was alcohol (45.87%). These compounds are known to have effects as hepatoprotective agents through antioxidant, anti-inflammatory, and anti-fibrotic activities that can reduce hepatic oxidative stress as an early trigger of cancer development. Through molecular docking, MOSEIL and MOLP showed a lower level of binding affinity when compared to TGF-β1 control drugs such as metformin. This data implies MOSEIL and MOLP have a strong interaction to TGF-β1 than the control drug. The therapeutic potential of the hepatoprotective properties of MOSEIL and MOLP makes them one of the most-promising therapeutic agents in the initial step of renewable cancer treatment therapy.

Item Type:Article
Uncontrolled Keywords:bioactive characterization, Hepatocellular carcinoma, molecular docking, Moringa oleifera, transforming Growth Factor β-1
Subjects:Q Science > Q Science (General)
Divisions:Science
ID Code:105338
Deposited By: Yanti Mohd Shah
Deposited On:24 Apr 2024 06:08
Last Modified:24 Apr 2024 06:08

Repository Staff Only: item control page