Blending Of Moringa oleifera into biodegradable polycaprolactone/silver electrospun membrane for hemocompatibility improvement

Abstract

Moringa oleifera (M. oleifera) has been utilized in conventional drug to treat illnesses associated with inflammation and wound healing. However, its utilization in tissue engineering application has not been explored yet. Reliable materials and carrier system are required to blend M. oleifera for sustainable therapeutic purpose. In this study, M. oleifera leave extracts were incorporated into electrospun polycaprolactone (PCL) nanofibrous membrane at various concentrations (19 wt%, 39 wt% and 59 wt%). A standard composition of 1 wt% silver nanoparticles (AgNPs) was also included to improve the membrane performance. The membranes were characterized with SEM–EDX, AFM and ATR-FTIR analyses. Further performances on the wettability, thermal, antibacterial and biocompatibility of the membranes were detailed assessed. The PCL/M. oleifera/AgNPs (PMA) membranes were viewed with smaller nanofiber diameter, greater surface roughness and higher wettability following the incorporation of M. oleifera. The notable appearances of C=C and N–O functional groups were observed on the PMA membranes with the composition of less than 1 wt% Ag. The PMA membranes were clarified thermally stable with a single step thermal decomposition. The antibacterial efficacy of the PMA membranes was prominent on Staphylococcus aureus (S. aureus) than Escherichia coli (E. coli). Importantly, the PMA membranes have enhanced the human platelet coagulant behavior significantly (p = 0.05), up to 39% for the PMA-3. The membranes are considered hemocompatible with a hemolysis index of = 5%. These results demonstrate that the PMA electrospun membranes have a potential to be used in tissue engineering application due to the therapeutic properties of M. oleifera.