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Development of methyl ester antibody-based competitive indirect ELISA for quantitative detection of Mitragynine in human urine

Mustafa, Radhiahtul Raehan and Sukor, Rashidah and Mohd. Nor, Siti Mariam and Saari, Nazamid and Mohsin, Aliah Zannierah (2023) Development of methyl ester antibody-based competitive indirect ELISA for quantitative detection of Mitragynine in human urine. ACS Omega, 8 (50). pp. 47412-47426. ISSN 2470-1343

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Official URL: http://dx.doi.org/10.1021/acsomega.3c02734

Abstract

Mitragynine is the main psychoactive compound of Mitragyna speciosa Korth. (kratom). This alkaloid could render psychotropic effects and is often misused as a substitute for commercial drugs. Nowadays, the increasing popularity of kratom has led to the development of a rapid and effective detection method. The detection of mitragynine in a biological sample such as urine requires a highly sensitive and specific method due to the complex nature of mitragynine in urine. Enzyme-linked immunosorbent assay (ELISA) is well known as a rapid screening method for biological samples. In this study, a competitive indirect ELISA was successfully developed using MG-22-OCH3 IgG as a detection antibody for mitragynine in human urine. The mitragynine immunoassay showed a limit of detection and a limit of quantification of 0.412 and 1.25 µg/mL, respectively. The measurement range was between 0.01 and 100.0 µg/mL, with a minimal inhibition (IC50) value of 0.152 µg/mL. The developed ELISA was validated using a gold method such as high-performance liquid chromatography-mass spectrometry (HPLC-MS). The percentage of recovery and the coefficient of variation (CV) for the ELISA and LCMS/MS analyses were 84.0-95.70%, 99.20-112.0%, 7.69-9.78%, and 2.86-6.62%, respectively. This indicates that the developed ELISA is a reliable method that can be used as a rapid approach for quantifying mitragynine content in biological samples.

Item Type:Article
Uncontrolled Keywords:Mitragyna speciosa Korth, Enzyme-linked immunosorbent assay (ELISA), high-performance liquid chromatography-mass spectrometry (HPLC-MS)
Subjects:H Social Sciences > H Social Sciences (General)
Divisions:Islamic Civilisation
ID Code:104866
Deposited By: Widya Wahid
Deposited On:25 Mar 2024 09:08
Last Modified:25 Mar 2024 09:08

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