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Genome mining of Pseudomonas spp. hints towards the production of under-pitched secondary metabolites

Shahid, Izzah and Han, Jun and Hanook, Sharoon and Borchers, Christoph H. and El Enshasy, Hesham Ali and Mehnaz, Samina (2023) Genome mining of Pseudomonas spp. hints towards the production of under-pitched secondary metabolites. 3 Biotech, 13 (6). pp. 1-13. ISSN 2190-572X

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Official URL: http://dx.doi.org/10.1007/s13205-023-03607-x

Abstract

The recent advances in omics and computational analysis have enabled the capacity to identify the exclusive strain-specific metabolites and novel biosynthetic gene clusters. This study analyzed eight strains of P. aurantiaca including GS1, GS3, GS4, GS6, GS7, FS2, ARS38, PBSt2, one strain of P. chlororaphis RP4, one strain of P. aeruginosa (At1RP4), and one strain of P. fluorescens (RS1) for the production of rhamnolipids, quorum-sensing signals, and osmolytes. Seven rhamnolipid derivatives were variably detected in fluorescent pseudomonads. These rhamnolipids included Rha-C10-C8, Rha-Rha-C10-C10, Rha-C10-C12db, Rha-C10-C10, Rha-Rha-C10-C12, Rha-C10-C12, and Rha-Rha-C10-C12db. Pseudomonas spp. also showed the variable production of osmoprotectants including N-acetyl glutaminyl glutamine amide (NAGGN), betaine, ectoine, and trehalose. Betaine and ectoine were produced by all pseudomonads, however, NAGGN and trehalose were observed by five and three strains, respectively. Four strains including P. chlororaphis (RP4), P. aeruginosa (At1RP4), P. fluorescens (RS1), and P. aurantiaca (PBSt2) were exposed to 1– 4% NaCl concentrations and evaluated for the changes in phenazine production profile which were negligible. AntiSMASH 5.0 platform showed 50 biosynthetic gene clusters in PB-St2, of which 23 (45%) were classified as putative gene clusters with ClusterFinder algorithm, five (10%) were classified as non-ribosomal peptides synthetases (NRPS), five (10%) as saccharides, and four (8%) were classified as putative fatty acids. The genomic attributes and comprehensive insights into the metabolomic profile of these Pseudomonas spp. strains showcase their phytostimulatory, phyto-protective, and osmoprotective effects of diverse crops grown in normal and saline soils.

Item Type:Article
Uncontrolled Keywords:Biosynthetic gene clusters, Genome mining, Osmolytes, Pseudomonas aurantiaca, Pyrrolnitrin, Rhamnolipids
Subjects:T Technology > TP Chemical technology
Divisions:Chemical and Energy Engineering
ID Code:104852
Deposited By: Widya Wahid
Deposited On:25 Mar 2024 09:03
Last Modified:30 Jun 2024 00:45

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