Ramanathan, Santheraleka and Gopinath, Subash C. B. and Ismail, Zool Hilmi and Subramaniam, Sreeramanan (2022) Nanodiamond conjugated SARS-CoV-2 spike protein: electrochemical impedance immunosensing on a gold microelectrode. Microchimica Acta, 189 (6). pp. 1-14. ISSN 0026-3672
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Official URL: http://dx.doi.org/10.1007/s00604-022-05320-7
Abstract
A promising immunosensing strategy in diagnosing SARS-CoV-2 is proposed using a 10-µm gap-sized gold interdigitated electrode (AuIDE) to target the surface spike protein (SP). The microelectrode surface was modified by (3-glycidyloxypropyl) trimethoxysilane to enforce the epoxy matrix, which facilitates the immobilization of the anti-SP antibody. The immunosensing performance was evaluated by integrating a nanosized (~ 10 nm) diamond-complexed SP as a target. The proposed immunoassay was quantitatively evaluated through electrochemical impedance spectroscopy (EIS) with the swept frequency from 0.1 to 1 MHz using a 100 mVRMSAC voltage supply. The immunoassay performed without diamond integration showed low sensitivity, with the lowest SP concentration measured at 1 pM at a determination coefficient of R2 = 0.9681. In contrast, the nanodiamond-conjugated SP on the immunosensor showed excellent sensitivity with a determination coefficient of R2 = 0.986. SP detection with a nanodiamond-conjugated target on AuIDE reached the low limit of detection at 189 fM in a linear detection range from 250 to 8000 fM. The specificity of the developed immunosensor was evaluated by interacting influenza-hemagglutinin and SARS-CoV-2-nucleocapsid protein with anti-SP. In addition, the authentic interaction of SP and anti-SP was validated by enzyme-linked immunosorbent assay.
Item Type: | Article |
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Uncontrolled Keywords: | biosensor, COVID-19, respiratory virus, spike protein |
Subjects: | Q Science > Q Science (General) T Technology > TK Electrical engineering. Electronics Nuclear engineering T Technology > TP Chemical technology |
Divisions: | Chemical and Energy Engineering |
ID Code: | 103293 |
Deposited By: | Yanti Mohd Shah |
Deposited On: | 31 Oct 2023 01:39 |
Last Modified: | 31 Oct 2023 01:39 |
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