Kaushal, Jyoti and Arya, Shailendra Kumar and Khatri, Madhu and Singh, Gursharan and Wan Azelee, Nur Izyan and Rajagopal, Rajinikanth and Chang, Soon Woong and Ravindran, Balasubramani and Awasthi, Mukesh Kumar (2022) Efficacious bioconversion of waste walnut shells to xylotetrose and xylopentose by free xylanase (Xy) and MOF immobilized xylanase (Xy-Cu-BTC). Bioresource Technology, 357 (NA). pp. 1-5. ISSN 0960-8524
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Official URL: http://dx.doi.org/10.1016/j.biortech.2022.127374
Abstract
This study uses a cost effective and efficient method for production of higher DP (degree of polymerization) Xylooligosaccharides (XOS) from xylan extracted from the waste walnut shells. Copper based metal organic framework (Cu-BTC MOF) was prepared for immobilization of free xylanase (Xy) enzyme by green synthesis method. Both free and immobilized xylanase (Xy-Cu-BTC) were able to cause the bioconversion of xylan (87.4% yield) into XOS. Predominant production of xylotetrose (X4) and xylopentose (X5) was observed for both the methods. Percentage XOS conversion for free enzyme (Xy) was found to be 4.1% X4 and 60.57% X5 whereas these values increased in case of immobilized system where 11.8% X4 and 64.2% X5 were produced. Xylose production was minute in case of immobilized xylanase 0.88% which makes it a better method for XOS production free from xylose interference. Xy-Cu-BTC MOF can hence be used as an attractive alternative for pure XOS production.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Hemicellulosic, Immobilization, Metal organic framework, Xylanase, Xylooligosaccharides (XOS), Xylopentose, Xylotetrose |
| Subjects: | T Technology > TP Chemical technology |
| Divisions: | Chemical and Energy Engineering |
| ID Code: | 101249 |
| Deposited By: | Widya Wahid |
| Deposited On: | 01 Jun 2023 10:10 |
| Last Modified: | 13 Nov 2023 06:18 |
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